Filming Dynamic Processes of Proteins by High-Speed Afm

Filming Biomolecular Processes by High-Speed Atomic Force Microscopy

Microscopy Toshio Ando,*,†,‡,§ Takayuki Uchihashi,†,‡,§ and Simon Scheuring †Department of Physics, and ‡Bio-AFM Frontier Research Center, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan CREST, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi 332-0012, Japan U1006 INSERM/Aix-Marseille Universite,́ Parc Scientifique et Technologique de Luminy Bat̂iment Inserm TPR2 bloc 5, 1...

Nanovisualization of Proteins in Action Using High-Speed AFM

The dynamics of proteins, such as their conformational change and dynamic interplay with interaction partners, is crucial to their biological functions. To reveal the dynamic behavior of proteins, single-molecule approaches are indispensable because the molecules behave in an unsynchronized manner, which makes it difficult to monitor their dynamics by ensemble average experiments. Fluorescence ...

High-speed AFM imaging.

Proteins are dynamic in nature and function at the single molecule level. To achieve a straightforward and in-depth understanding of their underlying functional mechanism, we need to directly observe protein molecules at work at high resolution, without the use of protein-attached markers. To realize such objectives, high-speed atomic force microscopy (HS-AFM) has been developed and recently it...

Chaperonin GroEL-GroES action captured by high-speed AFM

High-speed multicolor microscopy of repeating dynamic processes.

Images of multiply labeled fluorescent samples provide unique insights into the localization of molecules, cells, and tissues. The ability to image multiple channels simultaneously at high speed without cross talk is limited to a few colors and requires dedicated multichannel or multispectral detection procedures. Simpler microscopes, in which each color is imaged sequentially, produce a much l...